Li Yanwei

Personal profile:

In the past five years, he has hosted one National Natural Science Fund, one post-doctoral science fund, one natural science fund of Guangdong Province, and two national natural science funds and two national science and technology support plan projects. He has published 38 academic papers, including 35 papers in SCI, 1 fishery industry standard of the Ministry of Agriculture, 1 monograph, and 1 Guangdong Science and Technology Award.

Award, honorary title:

“Study on the biology and comprehensive prevention and control technology of the cryptic nucleus” won the second prize of the 2014 Guangdong Science and Technology Award (6/10).

Research areas:

The research field is the research and development of streptococcosis and stimulating cryptospirosis vaccines, which are harmful to China's fish farming industry, and the immune mechanism of fish disease resistance. The specific research directions are as follows: 
1. Fish TLR signaling pathway gene function the study. A large number of TLRs and their signaling pathway genes were identified, and their structural features and stimulating the expression of these genes were analyzed. The functional studies were carried out, and the fish IRAK4 and TAK1 were first discovered. Negative regulation of the -κB signal. 
2. Research on immune prevention and control technology for important diseases of fish. The research team carried out the development of a vaccine to stimulate the Cryptocaryon sinensis, the tilapia S. agalactiae vaccine, and the ovum-shaped S. faecium whole-cell vaccine, and evaluated the regulation of vaccine immunity against host disease resistance and immunity. effect. The screening of fish-stimulated Cryptocaryon irritans and Streptococcus immunogenic proteins was carried out to evaluate the potential of good immunogenic proteins as candidate antigens for subunit vaccines, laying the foundation for the development of subunit vaccines. The tilapia streptococci-free whole-bacteria inactivated vaccine was approved by the Ministry of Agriculture for clinical trials of veterinary biological products in May 2015 (the tilapia streptococci inactivated vaccine (THN strain) clinical trial, approval No.: 2015030).

Research projects:

1. National Natural Science Foundation of China Youth Science Foundation Project (31302208), Grouper TLR9 and TLR21 Signaling Pathway and Its Resistance Mechanism Research, 2014.01-2016.12, Research and Hosting; 
   2. Guangdong Provincial Natural Science Foundation Doctoral Research Launch Project (S2013040016268) , Grouper TLR9 and TLR21 ligand recognition function research, 2013.10-2015.10, completed, hosted; 
   3. Postdoctoral Science Foundation Funded Project (2012M521642), crystal structure study of important functional domains of grouper TLR9/21 , 2012.11-2014.11, completed, presided over; 
   4. National Natural Science Foundation of China (31172443), research on the natural resistance mechanism of the yellow-spotted bluefish to stimulate the Cryptocaryon, 2012.01 - 2015.12, completed, participated; 
   5. The National Natural Science Foundation of China (30871934), a study on the immune response of seawater fish induced by Cryptocaryon infection, 2009.01 - 2011.12, completed and participated.

Published papers:

1. Li YW, Zhao F, Mo ZQ, Luo XC, Li AX, Dan XM. Characterization, expression, and functional study of IRAK-1 from grouper, Epinephelus coioides. Fish Shellfish Immunol. 2016 56:374-81.
   2.Li YW, Xu DD, Li X, Mo ZQ, Luo XC, Li AX, Dan XM. Identification and characterization of three TLR1 subfamily members from the orange-spotted grouper, Epinephelus coioides. Dev Comp Immunol. 2016 61:180-9.
   3.Jiang B, Li YW, Abdullahi AY, Dan XM, Gong H, Ke QZ, Li AX, Li GQ. Placemat and rotational culturing: A novel method to control Cryptocaryon irritans infection by removing tomonts. Aquaculture. 2016 459:84-8.
   4.Zhang J, Wu XY, Li YW, Xie MQ, Li AX. The complete mitochondrial genome of Tetrancistrum nebulosi (Monogenea: Ancyrocephalidae). Mitochondrial DNA Part A. 2016 27:22-3.
   5.Su YL, Feng J, Li YW, Bai JS, Li AX. Development of a quantitative PCR assay for monitoring Streptococcus agalactiae colonization and tissue tropism in experimentally infected tilapia. J Fish Dis. 2016 39:229-38.
   6.Li YW, Wang Z, Mo ZQ, Li X, Luo XC, Dan XM, Li AX. Grouper (Epinephelus coioides) MyD88 and Tollip: Intracellular localization and signal transduction function. Fish Shellfish Immunol. 2015 42:153-8.
   7.Li YW, Li X, Wang Z, Mo ZQ, Dan XM, Luo XC, Li AX. Orange-spotted grouper Epinephelus coioides Tak1: molecular identification, expression analysis and functional study. Journal of Fish Biology. 2015 86:417-30.
   8.Mo ZQ, Li YW, Zhou L, Li AX, Luo XC, Dan XM. Grouper (Epinephelus coioides) IL-34/MCSF2 and MCSFR1/MCSFR2 were involved in mononuclear phagocytes activation against Cryptocaryon irritans infection. Fish Shellfish Immunol. 2015 43:142-9.
   9.Mo ZQ, Chen RA, Li YW, Huang XZ, Li AX, Luo XC, Dan XM. Characterization and expression analysis of two novel CCR6 chemokine receptors and their three potential ligands CCL20Ls of grouper (Epinephelus coioides) post Cryptocaryon irritans infection. Fish Shellfish Immunol. 2015 47:280-8.
   10.Mai YZ, Li YW, Li RJ, Li W, Huang XZ, Mo ZQ, Li AX. Proteomic analysis of differentially expressed proteins in the marine fish parasitic ciliate Cryptocaryon irritans. Vet Parasitol. 2015 211:1-11.
   11.Zhou LL, Dan XM, Li YW, Li XX, Chen XL, Li Y, Gan L. Effect of reducing 3.2% dietary protein level on the growth performance and immunity of Nile tilapia (Oreochromis niloticus) with supplementation of multi amino acids. Iranian Journal of Fisheries Sciences. 2015 14:997-1009.
   12.Zhao F, Li YW*, Pan HJ, Shi CB, Luo XC, Li AX, Wu SQ. TAK1-binding proteins (TAB1 and TAB2) in grass carp (Ctenopharyngodon idella): Identification, characterization, and expression analysis after infection with Ichthyophthirius multifiliis. Fish & Shellfish Immunology. 2014 38:389-99.(*通讯作者)
   13.Li YW, Mo XB, Zhou L, Li X, Dan XM, Luo XC, Li AX. Identification of IRAK-4 in grouper (Epinephelus coioides) that impairs MyD88-dependent NF-κB activation. Developmental & Comparative Immunology. 2014 45:190-7.
   14.Li YW, Liu L, Huang PR, Fang W, Luo ZP, Peng HL, Wang YX, Li AX. Chronic streptococcosis in Nile tilapia, Oreochromis niloticus (L.), caused by Streptococcus agalactiae. Journal of Fish Diseases. 2014 37:757-63.
   15.Li YW, Li X, Xiao XX, Zhao F, Luo XC, Dan XM, Li AX. Molecular characterization and functional analysis of TRAF6 in orange-spotted grouper (Epinephelus coioides). Developmental & Comparative Immunology. 2014 44:217-25.
   16.Zhang X1, Li YW1, Mo ZQ, Luo XC, Sun HY, Liu P, Li AX, Zhou SM, Dan XM. Outbreak of a novel disease associated with Vibrio mimicus infection in fresh water cultured yellow catfish, Pelteobagrus fulvidraco. Aquaculture. 2014 432:119-24. (1共同第一作者)
   17.Yi T1, Li YW1, Liu L, Xiao XX, Li AX. Protection of Nile tilapia (Oreochromis niloticus L.) against Streptococcus agalactiae following immunization with recombinant FbsA and α-enolase. Aquaculture. 2014 428–429:35-40. (1共同第一作者)
   18.Liu L1, Li YW1, He RZ, Xiao XX, Zhang X, Su YL, et al. Outbreak of Streptococcus agalactiae infection in barcoo grunter, Scortum barcoo (McCulloch & Waite), in an intensive fish farm in China. Journal of Fish Diseases. 2014 37:1067-72. (1共同第一作者)
   19.Zhao F, Li YW, Pan HJ, Shi CB, Luo XC, Li AX, Wu SQ. TAK1-binding proteins (TAB1 and TAB2) in grass carp (Ctenopharyngodon idella): Identification, characterization, and expression analysis after infection with Ichthyophthirius multifiliis. Fish Shellfish Immunol. 2014 38:389-99.
   20.Huang XZ, Li YW, Mai YZ, Luo XC, Dan XM, Li AX. Molecular cloning of NCCRP-1 gene from orange-spotted grouper (Epinephelus coioides) and characterization of NCCRP-1+ cells post Cryptocaryon irritans infection. Dev Comp Immunol. 2014 46:267-78.
   21.Yin F, Gong QY, Li YW, Dan XM, Sun P, Gao QX, Shi ZH, Peng SM, Li AX. Effects of Cryptocaryon irritans infection on the survival, feeding, respiratory rate and ionic regulation of the marbled rockfish Sebastiscus marmoratus. Parasitol. 2014 141:279-86.
   22.Zhang J, Wu XY, Li YW, Zhao M, Xie MQ, Li AX. The complete mitochondrial genome of Neobenedenia melleni (Platyhelminthes: Monogenea): mitochondrial gene content, arrangement and composition compared with two Benedenia species. Mol Biol Rep. 2014 41:6583-9.
   23.Zhao F, Li YW, Pan HJ, Wu SQ, Shi CB, Luo XC, Li AX. Grass carp (Ctenopharyngodon idella) TRAF6 and TAK1: Molecular cloning and expression analysis after Ichthyophthirius multifiliis infection. Fish Shellfish Immunol. 2013 34:1514-23.
   24.Zhao F, Li YW, Pan HJ, Shi CB, Luo XC, Li AX, Wu SQ. Expression profiles of toll-like receptors in channel catfish (Ictalurus punctatus) after infection with Ichthyophthirius multifiliis. Fish Shellfish Immunol. 2013 35:993-7.
   25.Dan XM, Zhong ZP, Li YW, Luo XC, Li AX. Cloning and expression analysis of grouper (Epinephelus coioides) M-CSFR gene post Cryptocaryon irritans infection and distribution of M-CSFR+ cells. Fish Shellfish Immunol. 2013 35:240-8.
   26.Dan XM, Zhang TW, Li YW, Li AX. Immune responses and immune-related gene expression profile in orange-spotted grouper after immunization with Cryptocaryon irritans vaccine. Fish Shellfish Immunol. 2013 34:885-91.
   27.Li YW, Luo XC, Dan XM, Qiao W, Huang XZ, Li AX. Molecular cloning of orange-spotted grouper (Epinephelus coioides) TLR21 and expression analysis post Cryptocaryon irritans infection. Fish & Shellfish Immunology. 2012 32:476-81.
   28.Hao J, Li YW, Xie MQ, Li AX. Molecular cloning, recombinant expression and antibacterial activity analysis of hepcidin from Simensis crocodile (Crocodylus siamensis). Comp Biochem Physiol B Biochem Mol Biol. 2012 163:309-15.
   29.Li YW, Luo XC, Dan XM, Huang XZ, Qiao W, Zhong ZP, Li AX. Orange-spotted grouper (Epinephelus coioides) TLR2, MyD88 and IL-1β involved in anti-Cryptocaryon irritans response. Fish & Shellfish Immunology. 2011 30:1230-40.
   30.Li YW, Dan XM, Zhang TW, Luo XC, Li AX. Immune-related genes expression profile in orange-spotted grouper during exposure to Cryptocaryon irritans. Parasite Immunology. 2011 33:679-987.

Publication of monographs and textbooks:

Graphical operation diagram of quarantine experiment for aquatic fingerlings, 2014, Guangdong Science and Technology Press, participated in the compilation.